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In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

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07:31 min

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October 08, 2017

DOI:

10.3791/56273-v

DOI:
10.3791/56273-v

•

07:31 min
October 08, 2017

•

2 Views
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1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Summary

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This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.

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In Vivo TrackingHuman Adipose-derived Mesenchymal Stem CellsRat Knee Osteoarthritis ModelFluorescent Lipophilic Membrane DyeDiDStem Cell PersistenceBiodistributionRegenerative MedicineAnimal ModelCell LabelingIn Vivo Imaging

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Cite this Article

Li, M., Hao, M., Jiang, D., Chen, Y., Wang, W. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J. Vis. Exp. (128), e56273, doi:10.3791/56273 (2017).

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