Journal
/
Entwicklungsbiologie
/
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

8,842 Views

•

07:31 min

•

October 08, 2017

DOI:

10.3791/56273-v

DOI:
10.3791/56273-v

•

07:31 min
October 08, 2017

•

2 Views
, , , ,
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Summary

Automatically generated

This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.

Explore More Videos

In Vivo TrackingHuman Adipose-derived Mesenchymal Stem CellsRat Knee Osteoarthritis ModelFluorescent Lipophilic Membrane DyeDiDStem Cell PersistenceBiodistributionRegenerative MedicineAnimal ModelCell LabelingIn Vivo Imaging

Read Article

Cite this Article

Li, M., Hao, M., Jiang, D., Chen, Y., Wang, W. In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye. J. Vis. Exp. (128), e56273, doi:10.3791/56273 (2017).

Download .ris file

Copy

Share Video

.

Copy