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JoVE Journal Developmental Biology
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
 

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

Article DOI: 10.3791/56273-v • 07:31 min • October 8th, 2017
October 8th, 2017 •
*1,2, *1,2, 3, 4, 1,2
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics, Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital, Tongji University
* These authors contributed equally

章节

总结概括
Automatic Translation

This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.

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Keywords: In Vivo Tracking Human Adipose-derived Mesenchymal Stem Cells Rat Knee Osteoarthritis Model Fluorescent Lipophilic Membrane Dye DiD Stem Cell Persistence Biodistribution Regenerative Medicine Animal Model Cell Labeling In Vivo Imaging
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