Journal
/
Biologia do Desenvolvimento
/
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

DOI:
10.3791/56273-v

•

07:31 min

•

October 08, 2017

•

, , , ,
1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics,Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital,Tongji University

Capítulos

  • 00:05Título
  • 00:58Surgical Rat Knee Osteoarthritis (KOA) Induction
  • 02:36Human Mesenchymal Stem Cell Fluorescent Dye Labelling and In Vivo Fluorescent Imaging
  • 05:16Results: Representative Rat KOA Induction and haMSC Imaging Analyses
  • 06:49Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection.

Tags

Keywords: In Vivo TrackingHuman Adipose-derived Mesenchymal Stem CellsRat Knee Osteoarthritis ModelFluorescent Lipophilic Membrane DyeDiDStem Cell PersistenceBiodistributionRegenerative MedicineAnimal ModelCell LabelingIn Vivo Imaging

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/56273?article_type=v

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
check_url/pt/56273?article_type=v

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
check_url/pt/56273?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/pt/56273?article_type=v

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
check_url/pt/56273?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/pt/56273?article_type=v

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
check_url/pt/56273?article_type=v

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
check_url/pt/56273?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/pt/56273?article_type=v

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
check_url/pt/56273?article_type=v

Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch’s Membrane

Read Article