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Journal
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Biochemistry
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質量分析を用いた識別 RNA 結合領域のための試料作製
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
Please note that all translations are automatically generated.
Click here for the English version.
質量分析を用いた識別 RNA 結合領域のための試料作製
DOI:
10.3791/56004-v
•
10:52 min
•
September 28, 2017
•
Robert Warneford-Thomson
4
,
Chongsheng He
2
,
Simone Sidoli
3
,
Benjamin A. Garcia
3
,
Roberto Bonasio
2
1
Epigenetics Institute
,
University of Pennsylvania Perelman School of Medicine
,
2
Department of Cell and Developmental Biology
,
University of Pennsylvania Perelman School of Medicine
,
3
Department of Biochemistry and Biophysics
,
University of Pennsylvania Perelman School of Medicine
,
4
Graduate Group in Biochemistry and Biophysics
,
University of Pennsylvania Perelman School of Medicine
Chapters
00:05
Title
01:04
Crosslink of Protein-RNA Interactions in Live Cells
03:26
Isolation of Nuclei
04:27
Lysis of Nuclei and Trypsin Digestion
05:51
Desalting of Peptides
07:39
Removal of Crosslinked RNA
08:44
Results: RNA-binding Protein and RNA-binding Region Identification
10:04
Conclusion
Summary
Automatic Translation
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Automatic Translation
RNA 結合蛋白質を識別し、紫外線による光架橋と質量分析法を用いた生細胞におけるその RNA 結合領域をマップするためのプロトコルについて述べる。
Tags
Sample Preparation
Mass Spectrometry
RNA-binding Proteins
UV Cross-linking
4sU Labeling
Cell Fractionation
Immunoprecipitation
Protein-RNA Interactions
Epigenetics
Cell Culture
Mouse Embryonic Stem Cells
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