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Bereiding van de monsters voor de massa spectrometrie gebaseerde identificatie van RNA-bindende regio 's
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10:52 min
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September 28, 2017
DOI:
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine
Summary
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We beschrijven een protocol te identificeren van RNA-bindende proteïnen en kaart van de regio's hun RNA-bindende in levende cellen met behulp van UV-gemedieerde photocrosslinking en massaspectrometrie.
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Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).
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