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Probenvorbereitung für Masse Spektrometrie-basierte Identifikation der RNA-bindende Regionen
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10:52 min
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September 28, 2017
DOI:
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine
Summary
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Wir beschreiben ein Protokoll zur RNA-bindende Proteine und RNA-bindende Regionen in lebenden Zellen mit UV-vermittelten Photocrosslinking und Massenspektrometrie.
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Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).
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