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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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10:52 min
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September 28, 2017
DOI:
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine
Summary
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We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.
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Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).
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