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Preparación de muestras para identificación basada en espectrometría de masa de regiones de unión de ARN
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10:52 min
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September 28, 2017
DOI:
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine
Summary
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Se describe un protocolo para identificar proteínas RNA-que atan y mapa de sus regiones de unión de ARN en células vivas usando photocrosslinking mediada por la UV y espectrometría de masas.
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Cite this Article
Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).
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