We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.
Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).