Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Robert Warneford-Thomson; Chongsheng He; Simone Sidoli; Benjamin A. Garcia; Roberto Bonasio

doi:10.3791/56004

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生化学

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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生化学

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JoVE Journal 生化学

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

7,952 Views

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10:52 min

•

September 28, 2017

DOI:

10.3791/56004-v

DOI:

10.3791/56004-v

•

10:52 min

September 28, 2017

•

2 Views

Robert Warneford-Thomson^1,4, Chongsheng He^1,2, Simone Sidoli^1,3, Benjamin A. Garcia^1,3, Roberto Bonasio^1,2

¹Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, ²Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ³Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, ⁴Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

概要

We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

概要

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