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ヒト筋芽細胞の単離、筋分化の評価、および貯蔵操作カルシウムエントリー測定
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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ヒト筋芽細胞の単離、筋分化の評価、および貯蔵操作カルシウムエントリー測定

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9,856 Views

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10:45 min

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July 26, 2017

DOI:

10.3791/55918-v

DOI:
10.3791/55918-v

•

10:45 min
July 26, 2017

•

3 Views
, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Summary

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ここでは、成人の筋肉組織からのヒト筋芽細胞の純粋な集団を得るための方法を説明する。これらの細胞は、in vitro骨格筋分化を研究するため、特にCa 2+シグナル伝達に関与するタンパク質を研究するために使用される。

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Cite this Article

Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).

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