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Journal
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Developmental Biology
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人成肌细胞的分离,造血分化的评估和存储操作的钙进入测量
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Developmental Biology
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JoVE Journal
Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Please note that all translations are automatically generated.
Click here for the English version.
人成肌细胞的分离,造血分化的评估和存储操作的钙进入测量
DOI:
10.3791/55918-v
•
10:45 min
•
July 26, 2017
•
Thomas Laumonier*
1
,
Stéphane Koenig*
2
,
Sophie Saüc
3
,
Maud Frieden
1
Department of Orthopedic Surgery
,
Geneva University Hospitals & Faculty of Medicine
,
2
Department of Basic Neurosciences
,
Geneva Medical Center
,
3
Department of Cell Physiology and Metabolism
,
Geneva Medical Center
Chapters
00:05
Title
01:07
Dissociation Protocol
02:42
Antibody Staining and Cell Sorting
04:39
Transfection
05:57
Ca
2+
Imaging in Differentiated Cells and Image Analysis
08:50
Results: Myoblast Characterization and a SOCE Experiment
09:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种从成年肌肉组织中获得纯种人成肌细胞的方法。这些细胞用于研究
体外
骨骼肌分化,特别是研究参与Ca
2+
信号传导的蛋白质。
Tags
Human Myoblasts
Myogenic Differentiation
Store-operated Calcium Entry
Skeletal Muscle
Muscle Regeneration
Ionic Channels
Transcription Factors
Muscular Dystrophies
Trypsin
Cell Culture
Cell Isolation
Cell Staining
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