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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

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9,840 Views

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10:45 min

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July 26, 2017

DOI:

10.3791/55918-v

DOI:
10.3791/55918-v

•

10:45 min
July 26, 2017

•

3 Views
, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Summary

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Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

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Cite this Article

Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).

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