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JoVE Journal Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
 

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Article DOI: 10.3791/55918-v • 10:45 min • July 26th, 2017
July 26th, 2017 •
*1, *2, 2,3, 3
1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center
* These authors contributed equally

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Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

Tags

Isolation Of Human Myoblasts Myogenic Differentiation Assessment Store-operated Calcium Entry Measurement Muscle Regeneration Calcium Signaling Ionic Channels Transcription Factors Gene Functions Human Myogenic Differentiation Myogenic Stem Cells Muscular Dystrophies Trypsin Digestion Tissue Dissociation
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