/
/
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
9,856 Views
•
10:45 min
•
July 26, 2017
DOI:
Summary
Automatically generated
Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.
Read Article
Cite this Article
Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).
Copy