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Isolering af humane myoblaster, vurdering af myogen differentiering og butiksdrevet calciumindgangsmåling
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Isolering af humane myoblaster, vurdering af myogen differentiering og butiksdrevet calciumindgangsmåling

DOI:
10.3791/55918-v

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10:45 min

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July 26, 2017

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, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Chapters

  • 00:05Title
  • 01:07Dissociation Protocol
  • 02:42Antibody Staining and Cell Sorting
  • 04:39Transfection
  • 05:57Ca2+ Imaging in Differentiated Cells and Image Analysis
  • 08:50Results: Myoblast Characterization and a SOCE Experiment
  • 09:54Conclusion

Summary

Automatic Translation

Automatic Translation

Her beskriver vi en metode til opnåelse af en ren population af humane myoblaster fra voksen muskelvæv. Disse celler anvendes til at studere in vitro skelettmuskeldifferentiering og især at studere proteiner involveret i Ca 2+ -signalering.

Tags

Human MyoblastsMyogenic DifferentiationStore-operated Calcium EntrySkeletal MuscleMuscle RegenerationIonic ChannelsTranscription FactorsMuscular DystrophiesTrypsinCell CultureCell IsolationCell Staining

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