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来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
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Immunology and Infection
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JoVE Journal Immunology and Infection
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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来自新世界Zika病毒感染性克隆的重组病毒的救援和表征

DOI:
10.3791/55857-v

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07:35 min

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June 07, 2017

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, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Chapters

  • 00:05Title
  • 00:40Preparation of In Vitro Transcribed RNA
  • 02:54Rescue of Infectious Virus by Electroporation
  • 05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

该方案描述了从双质粒感染性cDNA克隆中恢复感染性Zika病毒。

Tags

Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

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