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Immunologie und Infektion
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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

9,560 Views

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07:35 min

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June 07, 2017

DOI:

10.3791/55857-v

DOI:
10.3791/55857-v

•

07:35 min
June 07, 2017

•

1 Views
, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Summary

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This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.

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Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

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Cite this Article

Weger-Lucarelli, J., Duggal, N. K., Brault, A. C., Geiss, B. J., Ebel, G. D. Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone. J. Vis. Exp. (124), e55857, doi:10.3791/55857 (2017).

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