/
/
Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
A subscription to JoVE is required to view this content. Sign in or start your free trial.
9,808 Views
•
08:49 min
•
October 09, 2011
DOI:
Summary
Automatically generated
We describe a molecular clone of maedi-visna virus that expresses GFP and is fully infectious. Replication of this virus can be detected by using fluorescence microscopy and flow cytometry.
Explore More Videos
Maedi-visna VirusLentivirusInterstitial PneumoniaEncephalitisTarget CellsMonocyte LineageCell TypesGreen Fluorescent ProteinEgfp GeneDUTPase GeneReplicationCAEVMolecular CloneIn VitroIn VivoPrimary Cell LineChoroid PlexusTransfectionPropagationInfectiousEGFP ExpressionPassagesTCID50Infected CellsCell TropismPathogenicity
Read Article
Cite this Article
Jónsson, S. R., Andrésdóttir, V. Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein. J. Vis. Exp. (56), e3483, doi:10.3791/3483 (2011).
Copy