Journal
/
Immunologie et infection
/
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

DOI:
10.3791/55857-v

•

07:35 min

•

June 07, 2017

•

, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Chapitres

  • 00:05Titre
  • 00:40Preparation of In Vitro Transcribed RNA
  • 02:54Rescue of Infectious Virus by Electroporation
  • 05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
  • 07:02Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.

Tags

Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Generation of Recombinant Influenza Virus from Plasmid DNA

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein

Rescue of Recombinant Newcastle Disease Virus from cDNA

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons

Zika Virus Specific Diagnostic Epitope Discovery

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Read Article