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肉腫細胞における間葉上皮移行の誘導
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Developmental Biology
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JoVE Journal Developmental Biology
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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肉腫細胞における間葉上皮移行の誘導

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9,257 Views

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11:42 min

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April 07, 2017

DOI:

10.3791/55520-v

DOI:
10.3791/55520-v

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11:42 min
April 07, 2017

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3 Views
, , , , , , , , , , , ,
1Department of Medicine,Duke University, 2Department of Bioengineering,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

Summary

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ここでは、マイクロRNA-200ファミリーメンバーとgrainyhead様2（GRHL2）の合成異所性発現に基づく肉腫細胞における間葉上皮移行（MET）を誘導する細胞培養方法を提示します。この方法は、癌の悪性度や治療上の表現型可塑性の生物学的影響をよりよく理解するために適しています。

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Cite this Article

Ware, K. E., Gilja, S., Xu, S., Shetler, S., Jolly, M. K., Wang, X., Bartholf Dewitt, S., Hish, A. J., Jordan, S., Eward, W., Levine, H., Armstrong, A. J., Somarelli, J. A. Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells. J. Vis. Exp. (122), e55520, doi:10.3791/55520 (2017).

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