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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

DOI:
10.3791/55520-v

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11:42 min

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April 07, 2017

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, , , , , , , , , , , ,
1Tıp Fakültesi,Duke University, 2Biyomühendislik Bölümü,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

Bölümler

  • 00:05Başlık
  • 01:01Lentiviral Transduction of GRHL2
  • 06:00Sorting for EGFP+ Cells by Flow Cytometry
  • 07:14Reverse Transfection of miR-200s
  • 09:03Results: Representative Analysis of Tranduction Induced MET
  • 11:10Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

Etiketler

Mesenchymal-epithelial TransitionSarcomaPhenotypic PlasticityGene Regulatory NetworksTherapeutic InterventionsHEK293T CellsTransfectionLentivirus Production

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