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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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JoVE Journal 発生生物学
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

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11:42 min

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April 07, 2017

DOI:

10.3791/55520-v

DOI:
10.3791/55520-v

•

11:42 min
April 07, 2017

•

3 Views
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1医学部,Duke University, 2生物工学科,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

概要

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We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

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Cite this Article

Ware, K. E., Gilja, S., Xu, S., Shetler, S., Jolly, M. K., Wang, X., Bartholf Dewitt, S., Hish, A. J., Jordan, S., Eward, W., Levine, H., Armstrong, A. J., Somarelli, J. A. Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells. J. Vis. Exp. (122), e55520, doi:10.3791/55520 (2017).

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