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Induzione di mesenchimali-epiteliale transizioni in cellule di sarcoma
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Developmental Biology
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JoVE Journal Developmental Biology
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Induzione di mesenchimali-epiteliale transizioni in cellule di sarcoma

italiano

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9,257 Views

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11:42 min

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April 07, 2017

DOI:

10.3791/55520-v

DOI:
10.3791/55520-v

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11:42 min
April 07, 2017

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3 Views
, , , , , , , , , , , ,
1Department of Medicine,Duke University, 2Department of Bioengineering,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

Summary

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Presentiamo qui un metodo di coltura cellulare per indurre transizioni mesenchimali-epiteliale (MET) in cellule di sarcoma basato su espressione ectopica combinata di microRNA-200 familiari e grainyhead-like 2 (GRHL2). Questo metodo è adatto per una migliore comprensione dell'impatto biologico della plasticità fenotipica sull'aggressività cancro e trattamenti.

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Cite this Article

Ware, K. E., Gilja, S., Xu, S., Shetler, S., Jolly, M. K., Wang, X., Bartholf Dewitt, S., Hish, A. J., Jordan, S., Eward, W., Levine, H., Armstrong, A. J., Somarelli, J. A. Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells. J. Vis. Exp. (122), e55520, doi:10.3791/55520 (2017).

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