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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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JoVE Revista Biología del desarrollo
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

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11:42 min

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April 07, 2017

DOI:

10.3791/55520-v

DOI:
10.3791/55520-v

•

11:42 min
April 07, 2017

•

3 Views
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1Department of Medicine,Duke University, 2Department of Bioengineering,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

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We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

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Ware, K. E., Gilja, S., Xu, S., Shetler, S., Jolly, M. K., Wang, X., Bartholf Dewitt, S., Hish, A. J., Jordan, S., Eward, W., Levine, H., Armstrong, A. J., Somarelli, J. A. Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells. J. Vis. Exp. (122), e55520, doi:10.3791/55520 (2017).

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