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Biologia do Desenvolvimento
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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

9,257 Views

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11:42 min

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April 07, 2017

DOI:

10.3791/55520-v

DOI:
10.3791/55520-v

•

11:42 min
April 07, 2017

•

3 Views
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1Department of Medicine,Duke University, 2Department of Bioengineering,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

Summary

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We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

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Ware, K. E., Gilja, S., Xu, S., Shetler, S., Jolly, M. K., Wang, X., Bartholf Dewitt, S., Hish, A. J., Jordan, S., Eward, W., Levine, H., Armstrong, A. J., Somarelli, J. A. Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells. J. Vis. Exp. (122), e55520, doi:10.3791/55520 (2017).

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