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CXCR6.Gfp 기자 마우스를 사용하여 건강하고 병에 걸린 간에서 면역 세포의 장기 생체 내에 다중 광자 현미경 이미징
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
DOI:

11:44 min

March 24, 2015

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Chapters

  • 00:05Title
  • 02:50Tracheotomy
  • 04:36Laparatomy
  • 05:50Sample Setup
  • 08:39Results: Intravital TPLSM with CCI4 Damaged Livers
  • 10:02Conclusion

Summary

Automatic Translation

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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