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Immunology and Infection

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JoVE Journal Immunology and Infection
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
 

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Article DOI: 10.3791/52607-v • 11:44 min • March 24th, 2015
March 24th, 2015 •
*1, *1, 1, 1, 1, 1, 1, 2, 3, 1, 4, 1
1Department of Medicine III, RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging", RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery, RWTH Aachen University, 4Institute for Pharmacology, RWTH University-Hospital Aachen
* These authors contributed equally

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Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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Keywords: Intravital Microscopy Multiphoton Imaging Liver Inflammation Immune Cells CXCR6.Gfp Reporter Mice Leukocyte Migration Liver Injury Carbon Tetrachloride
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