Journal
/
Immunology and Infection
/
CXCR6.Gfpレポーターマウスを使用して、健康で罹患肝臓における免疫細胞の長期生体内多光子顕微鏡イメージング
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
Please note that all translations are automatically generated. Click here for the English version.

CXCR6.Gfpレポーターマウスを使用して、健康で罹患肝臓における免疫細胞の長期生体内多光子顕微鏡イメージング

日本語

Automatically Generated

11,773 Views

•

11:44 min

•

March 24, 2015

DOI:

10.3791/52607-v

DOI:
10.3791/52607-v

•

11:44 min
March 24, 2015

•

4 Views
, , , , , , , , , , ,
1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

Summary

Automatically generated

Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

Explore More Videos

Intravital MicroscopyMultiphoton ImagingLiver InflammationImmune CellsCXCR6.Gfp Reporter MiceLeukocyte MigrationLiver InjuryCarbon Tetrachloride

Read Article

Cite this Article

Heymann, F., Niemietz, P. M., Peusquens, J., Ergen, C., Kohlhepp, M., Mossanen, J. C., Schneider, C., Vogt, M., Tolba, R. H., Trautwein, C., Martin, C., Tacke, F. Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice. J. Vis. Exp. (97), e52607, doi:10.3791/52607 (2015).

Download .ris file

Copy

Share Video

.

Copy