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Immunology and Infection
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Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

DOI:
10.3791/3504-v

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10:08 min

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January 07, 2012

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, ,
1Laboratory of Immune Regulation,Instituto Gulbenkian de Ciência

Chapters

  • 00:01Title
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Results

Summary

Automatic Translation

Automatic Translation

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

Tags

Intravital ImagingMouse Thymus2-photon MicroscopyImage AcquisitionOrgansStereotactic ToolsSurgical ProceduresCellular BehaviorsLive AnimalsEx Vivo Imaging AcquisitionSurgical ProcedureOrgan HolderFoxp3+ CellsRegulatory T CellsNaturally-occurring Tregs (nTregs)Peripherally-converted Tregs (pTregs)In Vivo InteractionsPhenotype And PhysiologyNude Mice

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