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Largo Plazo intravital Multifotónica de imágenes de microscopía de células inmunitarias en sanos y enfermos de hígado utilizando ratones CXCR6.Gfp Reporter
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Immunology and Infection
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JoVE Journal Immunology and Infection
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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Largo Plazo intravital Multifotónica de imágenes de microscopía de células inmunitarias en sanos y enfermos de hígado utilizando ratones CXCR6.Gfp Reporter

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11,774 Views

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11:44 min

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March 24, 2015

DOI:

10.3791/52607-v

DOI:
10.3791/52607-v

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11:44 min
March 24, 2015

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, , , , , , , , , , ,
1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

Summary

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Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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Heymann, F., Niemietz, P. M., Peusquens, J., Ergen, C., Kohlhepp, M., Mossanen, J. C., Schneider, C., Vogt, M., Tolba, R. H., Trautwein, C., Martin, C., Tacke, F. Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice. J. Vis. Exp. (97), e52607, doi:10.3791/52607 (2015).

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