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Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice
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JoVE 杂志 免疫与感染
Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

11,773 Views

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11:44 min

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March 24, 2015

DOI:

10.3791/52607-v

DOI:
10.3791/52607-v

•

11:44 min
March 24, 2015

•

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, , , , , , , , , , ,
1Department of Medicine III,RWTH University-Hospital Aachen, 2IZKF Aachen Core Facility "Two-Photon Imaging",RWTH University-Hospital Aachen, 3Institute for Laboratory Animal Science & Experimental Surgery,RWTH Aachen University, 4Institute for Pharmacology,RWTH University-Hospital Aachen

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Stable intravital high-resolution imaging of immune cells in the liver is challenging. Here we provide a highly sensitive and reliable method to study migration and cell-cell-interactions of immune cells in mouse liver over long periods (about 6 hours) by intravital multiphoton laser scanning microscopy in combination with intensive care monitoring.

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Heymann, F., Niemietz, P. M., Peusquens, J., Ergen, C., Kohlhepp, M., Mossanen, J. C., Schneider, C., Vogt, M., Tolba, R. H., Trautwein, C., Martin, C., Tacke, F. Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice. J. Vis. Exp. (97), e52607, doi:10.3791/52607 (2015).

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