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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
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April 09, 2014
DOI:
1Biophysical Analytics, German Rheumatism Research Center,Leibniz Institute, 2Microscopy Core Facility,Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center,Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine
Summary
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High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
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Cseresnyes, Z., Oehme, L., Andresen, V., Sporbert, A., Hauser, A. E., Niesner, R. Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers. J. Vis. Exp. (86), e51135, doi:10.3791/51135 (2014).
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