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鶏の胚内の神経堤細胞と血管の二重標識は、ひよこGFP 神経管移植とカルボシアニンDyI注射を用いた
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
Please note that all translations are automatically generated. Click here for the English version.

鶏の胚内の神経堤細胞と血管の二重標識は、ひよこGFP 神経管移植とカルボシアニンDyI注射を用いた

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7,824 Views

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09:57 min

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May 28, 2015

DOI:

10.3791/52514-v

DOI:
10.3791/52514-v

•

09:57 min
May 28, 2015

•

7 Views
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1Birth Defects Research Centre,UCL Institute of Child Health, 2Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, 3Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Summary

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ここでは、血管内DiI注射と組み合わせたひよこGFP 神経管の種内移植を用いた神経堤細胞および血管の二重標識を報告する。この実験技術により、臓器形成中にNCC由来の(腸管)神経系と血管系の発達を同時に可視化し、研究することができます。

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Delalande, J., Thapar, N., Burns, A. J. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (99), e52514, doi:10.3791/52514 (2015).

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