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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
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Chapters
- 00:05Title
- 00:48Embryo Collection for Time-lapse Imaging
- 01:51Embryo Preparation
- 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
- 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
- 07:26Post-acquisition Processing
- 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
- 09:29Conclusion
A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.
