Journal
/
Developmental Biology
/
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

English

Automatically Generated

4,509 Views

•

08:47 min

•

May 15, 2020

DOI:

10.3791/61446-v

DOI:
10.3791/61446-v

•

08:47 min
May 15, 2020

•

3 Views
, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Summary

Automatically generated

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

Explore More Videos

Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

Read Article

Cite this Article

Hanafiah, A., Geng, Z., Wang, Q., Gao, Z. Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells. J. Vis. Exp. (159), e61446, doi:10.3791/61446 (2020).

Download .ris file

Copy

Share Video

.

Copy