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发育生物学
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Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection

DOI:
10.3791/52514-v

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09:57 min

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May 28, 2015

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, ,
1Birth Defects Research Centre,UCL Institute of Child Health, 2Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, 3Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Chapters

  • 00:05Title
  • 01:28Preparing the Host Embryo
  • 03:30Preparing the Donor Graft Tissue
  • 05:05Grafting the Tissue
  • 06:11Injecting Dil into Blood Vessels
  • 07:20Harvest Embryos for Sectioning or Wholemount Examination
  • 08:05Results: Dual Labeling in Chimeric Chick Embryos
  • 09:19Conclusion

Summary

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Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.

Tags

Neural Crest CellsBlood VesselsChicken EmbryosChickGFP Neural Tube GraftingCarbocyanine Dye DiI InjectionNervous SystemVascular SystemEnteric Nervous SystemChick ChimerasTransgenic ChickGFPQuail-chick Interspecies GraftingCell Development

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