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Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

DOI:
10.3791/60571-v

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11:17 min

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January 18, 2020

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, ,
1Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences,National Institutes of Health, 2NIH Stem Cell Unit, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapters

  • 00:05Title
  • 01:05Adaptation of Colony Type hESCs to Single-cell hESC Culture
  • 04:01Embryoid Body Formation and Differentiation
  • 05:44NPC Expansion and Cryopreservation
  • 06:41Differentiation of NPCs into Dopaminergic Neurons and Astrocytes
  • 08:54Results: In vitro Differentiation of Adapted Single-cell type hESCs
  • 10:34Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for the generation of a single-cell culture of human embryonic stem cells and their subsequent differentiation into neural progenitor cells. The protocol is simple, robust, scalable, and suitable for drug screening and regenerative medicine applications.

Tags

Human Embryonic Stem CellsNeural Progenitor CellsSingle-cell CultureEfficient DifferentiationScalable Culture SystemMulti-step DifferentiationNeural LineagesBasement Membrane MatrixCell DetachmentMTeSR-1 Medium

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