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Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection

Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection

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7,824 Views

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09:57 min

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May 28, 2015

DOI:

10.3791/52514-v

DOI:
10.3791/52514-v

•

09:57 min
May 28, 2015

•

7 Views
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1Birth Defects Research Centre,UCL Institute of Child Health, 2Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, 3Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Summary

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Here we report dual labeling of neural crest cells and blood vessels using chickGFP neural tube intraspecies grafting combined with intra-vascular DiI injection. This experimental technique allows us to simultaneously visualize and study development of the NCC-derived (enteric) nervous system and the vascular system, during organogenesis.

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Delalande, J., Thapar, N., Burns, A. J. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (99), e52514, doi:10.3791/52514 (2015).

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