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使用小鸡GFP 神经管移植和碳水化合物染料 DiI 注射，在鸡胚胎内对神经囊细胞和血管进行双重标记
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection
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使用小鸡GFP 神经管移植和碳水化合物染料 DiI 注射，在鸡胚胎内对神经囊细胞和血管进行双重标记

English

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7,764 Views

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09:57 min

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May 28, 2015

DOI:

10.3791/52514-v

DOI:
10.3791/52514-v

•

09:57 min
May 28, 2015

•

7 Views
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1Birth Defects Research Centre,UCL Institute of Child Health, 2Blizard Institute, Centre for Digestive Diseases,Queen Mary University of London, Barts and The London School of Medicine and Dentistry, 3Department of Clinical Genetics,Erasmus University Medical Center, Rotterdam

Summary

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在这里，我们报告神经波峰细胞和血管的双重标签使用小鸡GFP 神经管内部嫁接结合血管内DII注射。这种实验技术使我们能够同时在器官发生期间可视化和研究NCC衍生（肠）神经系统和血管系统的发展。

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Delalande, J., Thapar, N., Burns, A. J. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (99), e52514, doi:10.3791/52514 (2015).

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