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Photoactivated 단일 분자 추적을 사용하여 라이브 박테리아 세포에있는 단백질-DNA 상호 작용을 시각화
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
DOI:

16:21 min

March 10, 2014

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Chapters

  • 00:05Title
  • 01:32Cell Slide Preparation
  • 04:00Preparing Microscopy Data Acquisition
  • 06:43Acquiring Photo-activated Localization Microscopy (PALM) Movies
  • 08:16PALM Movie Analysis
  • 12:48Representative Results: Pol1-DNA Binding in Single Bacterial Cells
  • 15:53Conclusion

Summary

Automatic Translation

단일 분자 추적과 함께 Photoactivated 현지화 현미경 (PALM)은 허용 라이브 대장균 세포에서 단백질-DNA 상호 작용의 직접 관찰 및 정량화.

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