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Immunology and Infection
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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

English

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9,741 Views

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14:05 min

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September 19, 2013

DOI:

10.3791/51043-v

DOI:
10.3791/51043-v

•

14:05 min
September 19, 2013

•

1 Views
, , , , , ,
1Unité des Interactions Bactéries Cellules,Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry,ETH Zürich, 5Focal Area Infection Biology, Biozentrum,University of Basel

Summary

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Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.

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Listeria MonocytogenesBacterial PathogenIntracellular ParasitismCellular Immune ResponseInlCFluorescent LabelingMicroscopyHigh-throughput SiRNA ScreeningCellular Signaling Pathways

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Cite this Article

Kühbacher, A., Gouin, E., Mercer, J., Emmenlauer, M., Dehio, C., Cossart, P., Pizarro-Cerdá, J. Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes. J. Vis. Exp. (79), e51043, doi:10.3791/51043 (2013).

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