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Visualización de la proteína-DNA en células bacterianas en directo mediante fotoactivado Tracking Single-molécula
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
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Visualización de la proteína-DNA en células bacterianas en directo mediante fotoactivado Tracking Single-molécula

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17,582 Views

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16:21 min

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March 10, 2014

DOI:

10.3791/51177-v

DOI:
10.3791/51177-v

•

16:21 min
March 10, 2014

•

2 Views
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1Microbiology Unit, Department of Biochemistry,University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Summary

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Microscopía de localización fotoactivado (PALM) combinada con el seguimiento de una sola molécula permite la observación directa y la cuantificación de la proteína-DNA en células de E. coli vivas de Escherichia.

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Uphoff, S., Sherratt, D. J., Kapanidis, A. N. Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking. J. Vis. Exp. (85), e51177, doi:10.3791/51177 (2014).

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