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Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

17,570 Views

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16:21 min

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March 10, 2014

DOI:

10.3791/51177-v

DOI:
10.3791/51177-v

•

16:21 min
March 10, 2014

•

2 Views
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1Microbiology Unit, Department of Biochemistry,University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Summary

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Photoactivated localization microscopy (PALM) combined with single-molecule tracking allows direct observation and quantification of protein-DNA interactions in live Escherichia coli cells.

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Protein-DNA InteractionsLive Bacterial CellsPhotoactivated Single-molecule TrackingDNA ReplicationTranscriptionRepairChromosome OrganizationDNA-binding ProteinsPhotoactivated Localization Microscopy (PALM)Single-molecule TrackingDNA-binding EventsProtein ActivitySingle-cell Level

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Uphoff, S., Sherratt, D. J., Kapanidis, A. N. Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking. J. Vis. Exp. (85), e51177, doi:10.3791/51177 (2014).

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