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光活性化単一分子追跡を使用してライブ細菌細胞におけるタンパク質-DNA相互作用を可視化する
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
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光活性化単一分子追跡を使用してライブ細菌細胞におけるタンパク質-DNA相互作用を可視化する

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17,582 Views

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16:21 min

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March 10, 2014

DOI:

10.3791/51177-v

DOI:
10.3791/51177-v

•

16:21 min
March 10, 2014

•

2 Views
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1Microbiology Unit, Department of Biochemistry,University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Summary

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単一分子追跡と組み合わさ光活性化局在化顕微鏡（PALM）は、生大腸菌細胞におけるタンパク質-DNA相互作用の直接観察及び定量化を可能にする。

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Protein-DNA InteractionsLive Bacterial CellsPhotoactivated Single-molecule TrackingDNA ReplicationTranscriptionRepairChromosome OrganizationDNA-binding ProteinsPhotoactivated Localization Microscopy (PALM)Single-molecule TrackingDNA-binding EventsProtein ActivitySingle-cell Level

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Uphoff, S., Sherratt, D. J., Kapanidis, A. N. Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking. J. Vis. Exp. (85), e51177, doi:10.3791/51177 (2014).

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