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Visualizzare le interazioni proteina-DNA in cellule vive batteriche Uso fotoattivati ​​singola molecola di monitoraggio
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
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Visualizzare le interazioni proteina-DNA in cellule vive batteriche Uso fotoattivati ​​singola molecola di monitoraggio

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17,582 Views

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16:21 min

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March 10, 2014

DOI:

10.3791/51177-v

DOI:
10.3791/51177-v

•

16:21 min
March 10, 2014

•

2 Views
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1Microbiology Unit, Department of Biochemistry,University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Summary

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Fotoattivati ​​microscopia localizzazione (PALM) in combinazione con inseguimento singola molecola permette l'osservazione diretta e la quantificazione delle interazioni proteina-DNA in cellule di Escherichia coli vive.

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Cite this Article

Uphoff, S., Sherratt, D. J., Kapanidis, A. N. Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking. J. Vis. Exp. (85), e51177, doi:10.3791/51177 (2014).

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