/
/
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Title
- 01:17Preparation of THP-1 Macrophage Monolayer
- 02:59Preparation of Candida glabrata Cell Suspension
- 04:26Infection of THP-1 Macrophages with C. glabrata Cells
- 05:52Measurement of Phagocytosis Rate and Intracellular Replication via Colony Forming Unit Assay
- 07:20Monitoring of Intracellular Replication Using Confocal Laser Scanning Microscopy
- 09:07Representative Results
- 09:33Conclusion
The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.
