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Immunologie et infection
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Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
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Immunologie et infection
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Journal JoVE Immunologie et infection
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

11,460 Views

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13:47 min

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December 10, 2013

DOI:

10.3791/50625-v

DOI:
10.3791/50625-v

•

13:47 min
December 10, 2013

•

3 Views
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1Laboratory of Fungal Pathogenesis,Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent,Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium

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The current article outlines a protocol to establish an in vitro cell culture model system to study the interaction of a facultative intracellular human fungal pathogen Candida glabrata with human macrophages which will be a useful tool to advance our knowledge of fungal virulence mechanisms.

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In Vitro ModelTHP-1 MacrophagesCandida GlabrataIntracellular PathogenHost-pathogen InteractionMicrobial VirulenceCell CultureInfection ModelHigh-throughput Screening

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Cite this Article

Rai, M. N., Borah, S., Bairwa, G., Balusu, S., Gorityala, N., Kaur, R. Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages. J. Vis. Exp. (82), e50625, doi:10.3791/50625 (2013).

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