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Estabelecimento de um<em> In vitro</em> Sistema para estudar o comportamento intracelular de<em> Candida glabrata</em> Em humanos THP-1 macrófagos
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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

Estabelecimento de um<em> In vitro</em> Sistema para estudar o comportamento intracelular de<em> Candida glabrata</em> Em humanos THP-1 macrófagos

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11,460 Views

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13:47 min

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December 10, 2013

DOI:

10.3791/50625-v

DOI:
10.3791/50625-v

•

13:47 min
December 10, 2013

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3 Views
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1Laboratory of Fungal Pathogenesis,Centre for DNA Fingerprinting and Diagnostics, Andhra Pradesh, India, 2Current location: VIB Department for Molecular Biomedical Research, UGent,Fiers-Schell-Van Montagu Building, Technologiepark 927, B-9052 Ghent (Zwijnaarde), Belgium

Summary

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O presente artigo descreve um protocolo para estabelecer um sistema modelo de cultura de células in vitro para estudar a interação de um patógeno humano intracelular facultativo de fungos Candida glabrata com macrófagos humanos, que será uma ferramenta útil para aumentar o nosso conhecimento dos mecanismos de virulência de fungos.

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Cite this Article

Rai, M. N., Borah, S., Bairwa, G., Balusu, S., Gorityala, N., Kaur, R. Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages. J. Vis. Exp. (82), e50625, doi:10.3791/50625 (2013).

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